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R&D Systems
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R&D Systems
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R&D Systems
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Journal: Journal of the Endocrine Society
Article Title: Subclinically low BMD in young men is associated with compromised bone microarchitecture and lower lean mass
doi: 10.1210/jendso/bvag003
Figure Lengend Snippet: Biomarkers. Fig. 1 depicts all assessed biomarker concentrations. The total hip, femoral neck, or lumbar spine (Lower) group is depicted in black filled circles, while the control (Con) group is in clear squares. A, P1NP (pg/mL); B, alkaline phosphatase (ALP) (pg/mL); C, tartrate-resistant acid phosphatase 5b (TRAP5b) (U/L); D, sclerostin (pmol/L); E, β C-terminal cross-linked telopeptide of type I collagen (βCTX) (pmol/L); F, testosterone (ng/mL); G, estradiol (pg/mL); H, parathyroid hormone (PTH) (pg/mL); I, insulin-like growth factor-1 (IGF-1) (ng/mL); J, insulin-like growth factor-1 binding protein 5 (IGFBP-5) (ng/mL).
Article Snippet: Commercially available enzyme-linked immunosorbent assays were used to determine circulating concentrations of serum procollagen type I N-terminal propeptide (PINP; Novus catalog No. NBP2-76465, RRID:AB_3720306, 1:200 dilution, CV 5.3%, sensitivity 9.4 pg/mL), alkaline phosphatase (ALP; Novus catalog No. NBP2-68197, RRID:AB_3720307, CV 6.0%, sensitivity 46.9 pg/mL), tartrate-resistant acid phosphatase 5b (TRAP5b; Immunodiagnostic Systems catalog No. SB-TR201A, RRID:AB_3095995, CV 4.7%, sensitivity 0.4 U/L), sclerostin (Biomedica catalog No. BI-20492, RRID:AB_2894889, CV 6.4%, sensitivity 3.2 pmol/L), testosterone (Alpco Diagnostics catalog No. 11-TESHU-E01, RRID:AB_3720308, CV 4.2%, sensitivity 0.02 ng/mL), estradiol (Alpco Diagnostics catalog No. 11-ESTHU-E01, RRID:AB_2756385, CV 7.6%, sensitivity 10 pg/mL), insulin like growth factor-1 (IGF-1; Alpco Diagnostics catalog No. 22-IGFHU-E01, RRID:AB_2923276, 1:20 dilution, average CV 3.3%, assay sensitivity 9.4 pg/mL),
Techniques: Biomarker Discovery, Control, Binding Assay
Journal: Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society
Article Title: Growth cartilage expression of growth hormone/insulin-like growth factor I axis in spontaneous and growth hormone induced catch-up growth.
doi: 10.1016/j.ghir.2012.04.004
Figure Lengend Snippet: Fig. 1. Representative examples of in situ hybridization for IGF-I, IGFBP5 and IGF-IR mRNAs for the three studied groups: control animals (C group), animals submitted to the nox- ious stimulus (CR group), and animals submitted to the noxious stimulus treated with growth hormone (CRGH group). IGF-I: Insulin-like Growth Factor-I; IGFBP5: Insulin-like Growth Factor Binding Protein 5; IGF-IR: Insulin-like Growth Factor-I Receptor.
Article Snippet: Immunohistochemical staining was performed in PFA-fixed frontal sections (5 μm thick) of proximal tibiae, using IGF-I and
Techniques: In Situ Hybridization, Control, Binding Assay
Journal: Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society
Article Title: Growth cartilage expression of growth hormone/insulin-like growth factor I axis in spontaneous and growth hormone induced catch-up growth.
doi: 10.1016/j.ghir.2012.04.004
Figure Lengend Snippet: Fig. 2. Representative examples of immunohistochemistry for IGF-I and IGFBP5 for the three studied groups: control animals (C group), animals submitted to the noxious stimulus (CR group), and animals submitted to the noxious stimulus treated with growth hormone (CRGH group). A negative control is also presented. IGF-I: Insulin-like Growth Factor-I; IGFBP5: Insulin-like Growth Factor Binding Protein 5.
Article Snippet: Immunohistochemical staining was performed in PFA-fixed frontal sections (5 μm thick) of proximal tibiae, using IGF-I and
Techniques: Immunohistochemistry, Control, Negative Control, Binding Assay